Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: Hs68 cells were treated with different concentrations of FRA (0– 1,600 μg/ml) and its fractions (0–200 μg/ml) for 24 h and then cytotoxicity was performed ( n = 6). Data are presented as mean ± standard error ( n = 3). Significant differences were analyzed using one-way ANOVA and Dunnett's test. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: ( A ) The area and speed of Proliferation of Hs68 cells was measured through a 500 μm cell-free gap created by a culture insert placed in the center of a 35 mm μ-Dish (Ibidi, Germany). Measurements were made using a microscope every 6 h during 24-h incubation through real-time monitoring. Scale bar = 200 μm. ( B ) The wound area of Hs68 cells treated with 200 ppm of FRA-Bu was significantly narrowed compared to untreated cells. ( C ) The migration area expressed as a percentage based on the initial cell-free interval, increased significantly at 18 h of culture. The area area was measured in real-time using image j. Significant differences were analyzed using by unpaired t -test ( n = 3). Data are presented as mean ± standard error ( n = 3). * p < 0.05 compared with the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques: Microscopy, Incubation, Migration

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: The cell ratio in the G0/G1, S, and G2/M phases of proliferation on the FRA-Bu treated Hs68 cells was analyzed using Muse Cell Cycle Analyser. ( A ) A histogram of a representative experiment shows the effect of FRA-Bu on the cell cycle profile. ( B-C ) In particular, the treated cells showed a significant increase in the S phase of the cell cycle, the origin of DNA replication, after 18 hours of culture. Significant differences were analyzed using an unpaired t -test ( n = 3). Data are presented as mean ± standard error ( n = 3). * p < 0.05 compared to the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: The mRNA expressions of CCND1 , CDK4, CCND2, CDK2, CCNA1 , CCNA2 , and CCNB2 were measured by qPCR in Hs68 cells (treated or nontreated) after 18 and 24 h. Significant differences were analyzed using an unpaired t -test ( n = 3). Data are presented as mean ± standard error ( n = 3). * p < 0.05 compared to the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques: